P-site™ antibody service (Phosphorylation Site-Specific Antibody)

Post-translational modifications (PTM) play important roles in regulating the biological activity of a protein. Phosphorylatioin is one of the most common PTM carried out by the protein kinases. A properly designed P-site™-specific antibody allows one to monitor the kinase activation event without the use of radioisotope 32p.

Our team of scientists have derived a wealth of experience over the years by making hundreds of P-site™-specific antibodies. The design of the P-site™ epitope critically determines the outcome (yield, specificity and affinity) of the final antibody. Based on your individual project needs, our team will tailor the epitope design accordingly.

Call us also about other PTM-specific antibody services (e.g., hydroxylation, methylation, acetylation, neopeptide, etc.)

 

Yen-de-Zyn of P-site™ epitope

FREE

Synthesis of phosphorylated peptide:

  • HPLC high purity peptide will be used for immunization purposes
  • General grade peptide for ELISA and Affinity Purification purposes
  • Remaining amount of peptide will be provided to the investigator

$650

($25 per extra residue when exceeding an usual length of 15 residues, should epitope design call for, in unusual cases)

Synthesis of unphosphorylated peptide counterpart:

  • General grade peptide will be used for ELISA and affinity absorption purposes
  • Remaining amount of peptide will be provided to the investigator

$200

($15 per extra residue when exceeding an usual length of 15 residues, should epitope design call for, in unusual cases)

Carrier Protein Conjugations of:

  • HPLC-highly purified phosphorylated peptide to carrier protein KLH or THY for immunization,
  • General grade phosphorylated peptide to GIgG and
  • General grade unphosphorylated peptide counterpart to GIgG for ELISA
$250

P-site™ Regular™ Antibody production (2 Rabbits)

  • Regular™ Antibody Production regimen
  • 5 ml pre-immune serum per rabbit
  • 5 ml test bleed serum per rabbit
  • Test bleed ELISA of phosphorylated vs. unphosphorylated peptide
  • 50 ml final antiserum per rabbit
$800

Step1. Phosphopeptide-specific Affinity Purification of Antibody

  • Preparation of phospho-peptide conjugated affinity matrix
  • Separate purification of both rabbit antiserum
  • Affinity matrix will be provided to the investigator upon completion of project
$795

Step 2. Affinity Absorption of Step 1-purified Antibody to separate P-site-specific antibody from the cross-reactive antibody populations

  • Preparation of unphosphorylated peptide conjugated affinity matrix
  • Separate absorption of both Step 1 purified antibodies
  • ELISA of pre- and post-purified serum to ensure depletion of specific antibody and ELISA of purified and absorbed antibody preparations against phosphorylated and unphosphorylated peptides to ensure P-site™ specificity
  • SDS-PAGE of final antibody preparations
  • Affinity matrix will be provided to the investigator upon completion of project
$795
Total:
$3,490 $2,695

 

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